Insertion junction: CLIP2.043061_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.043061
Chromosome:chromosome 3
Location:3392312
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g166600 DIV147 (1 of 1) PTHR13366//PTHR13366:SF0 - MALARIA ANTIGEN-RELATED // HEAT REPEAT-CONTAINING PROTEIN 6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGCGACAGCGGTGCAGCATAGCGTGCACAGTGTCGACTCACTGAACAA
Internal bar code:CAGGCAAAGATGTACGCTTTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:66
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ACCATGAATGAGCTGTGCGA
Suggested primer 2:TCACATACACGTGGGAAGGC