Insertion junction: CLIP2.043061_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.043061
Chromosome:chromosome 3
Location:3392312
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g166600 DIV147 (1 of 1) PTHR13366//PTHR13366:SF0 - MALARIA ANTIGEN-RELATED // HEAT REPEAT-CONTAINING PROTEIN 6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTGCGGCCCCAACCGCAGACAACAAATGCAAACGCAAGCGGACCTGGC
Internal bar code:CAGGCAAAGATGTACGCTTTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:510
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TCACATACACGTGGGAAGGC
Suggested primer 2:ACCATGAATGAGCTGTGCGA