Insertion junction: CLIP2.043066_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.043066
Chromosome:chromosome 10
Location:4837699
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g453350 (1 of 1) PF00097//PF04536 - Zinc finger, C3HC4 type (RING finger) (zf-C3HC4) // TLP18.3, Psb32 and MOLO-1 founding proteins of phosphatase (TPM) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGTTGGGTGGAATTGGAGACGGCGGGGGTGGTACGGTAAGTGAGTTGG
Internal bar code:TTGAAAATGGCCTGCTTTTCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2870
LEAP-Seq percent confirming:97.1154
LEAP-Seq n confirming:101
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:104

Suggested primers for confirmation by PCR

Suggested primer 1:CTACCCCAATCCGATGGTCG
Suggested primer 2:GTCAGTTGCGAAGTCCTCCA