Insertion junction: CLIP2.043066_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.043066
Chromosome:chromosome 10
Location:4837699
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g453350 (1 of 1) PF00097//PF04536 - Zinc finger, C3HC4 type (RING finger) (zf-C3HC4) // TLP18.3, Psb32 and MOLO-1 founding proteins of phosphatase (TPM) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCGGAGATGCCGTGGAGCGCGGTGTGGTCAACATTGGGCTGGCGCTGGC
Internal bar code:TTGAAAATGGCCTGCTTTTCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2129
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GTCAGTTGCGAAGTCCTCCA
Suggested primer 2:CTACCCCAATCCGATGGTCG