| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.043076 |
| Chromosome: | chromosome 1 |
| Location: | 4156728 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g027750 | MAE7,MFT7 | MATE efflux family protein | intron |
| Cre01.g027764 | (1 of 11) K03327 - multidrug resistance protein, MATE family (TC.MATE, SLC47A, norM, mdtK, dinF) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCCCGTCAGGGTGCGCCCATGGCACCGACGCGGCTGCGTGGGCGTGTT |
| Internal bar code: | TACGTCTTTGTTTAGAAGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 385 |
| LEAP-Seq percent confirming: | 53.3333 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGAGTTTGAAGGTCCGG |
| Suggested primer 2: | ACGCTTGGATGCAGGGTTTA |