| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.043077 |
| Chromosome: | chromosome 12 |
| Location: | 2768447 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g504100 | PHX20,NFU2,PFH20 | Putative prolyl 4-hydroxylase; (1 of 26) 1.14.11.2 - Procollagen-proline dioxygenase / Protocollagen hydroxylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGAACACAGCGGCGCAGCGGCCTGAGGCGCAGGCGGTAGTCGCAGGCA |
| Internal bar code: | TGGTGATTGGGTTTCTCCAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCTGGGCTTCGGTTCAG |
| Suggested primer 2: | CTGCACCAACAAACTTGCGT |