| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.043079 |
| Chromosome: | chromosome 6 |
| Location: | 3724974 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278160 | PRL5 | (1 of 14) PTHR10334 - CYSTEINE-RICH SECRETORY PROTEIN-RELATED; Predicted extracellular protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTCCGAGTACCAGGCGTCCATGGCCGCTGTGATGGAGGGGTGGCCCA |
| Internal bar code: | GCAGGGTATTCATTTCTTGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 243 |
| LEAP-Seq percent confirming: | 41.1765 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACTGCTGGTGGGTAGGAG |
| Suggested primer 2: | AGCCTTGCATGGAAGCATCT |