Insertion junction: CLIP2.043080_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.043080
Chromosome:chromosome 7
Location:1647404
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g325350 KIN4-3,KIN10A,KIN10-1 Kinesin motor protein; (1 of 4) K10395 - kinesin family member 4/21/27 (KIF4_21_27) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCCGCCACTGTCCCACGCAGGGCCCTTTCCGCTCCTCGCCATCCCCTC
Internal bar code:TTCAGCGTCCAACCTAGGTCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1136
LEAP-Seq percent confirming:75.6098
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:TCAACCTGTCGCTCATGACC
Suggested primer 2:CTCATTGCGCAGTCTGCATC