Insertion junction: CLIP2.043090_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.043090
Chromosome:chromosome 10
Location:463854
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g420850 (1 of 20) IPR001190//IPR017448 - SRCR domain // SRCR-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACCCGAGCTTCCTTGCGCTCACCATTTGAGCAGATGACCGCTGCCAC
Internal bar code:GGTTCACACACACGGTTTTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2933
LEAP-Seq percent confirming:97.6378
LEAP-Seq n confirming:124
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:127

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGGGTGCCCGTTGTCTAG
Suggested primer 2:CATGGACGGATACTTCGCGA