| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.043091 |
| Chromosome: | chromosome 17 |
| Location: | 3315662 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g722500 | (1 of 2) K09660 - mannose-P-dolichol utilization defect 1 (MPDU1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGACTGAGGCCCTCCGCGGTGCCAGCGTTGCGGATGACCAGGATCTGT |
| Internal bar code: | AGACCTTTGTACTGATTTTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2417 |
| LEAP-Seq percent confirming: | 82.7586 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGATACGCGTGTGGCAAG |
| Suggested primer 2: | CAGCGAGGCTCAAATGCATC |