| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.043124 |
| Chromosome: | chromosome 13 |
| Location: | 2467687 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g801530 | (1 of 6) 2.7.11.21 - Polo kinase / Polo-like kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTACTCCTTTTTGGGGCTGAGCCTTCGAGGCTCGAGCCCACCTTTCTT |
| Internal bar code: | GGATGTCGGATTGGAAGGAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 203 |
| LEAP-Seq percent confirming: | 3.7037 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCCGACAACCCCTTGTAA |
| Suggested primer 2: | TACTACACCCTGCAGGACGA |