Insertion junction: CLIP2.043140_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.043140
Chromosome:chromosome 17
Location:238287
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g697500 P25 Alpha Domain and EF Hand Protein; (1 of 2) PTHR12932:SF9 - TPPP FAMILY PROTEIN CG45057 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGACAGGTTTGACATGGAACCATGTATGTGATTGCCCGCCATCCTGAGC
Internal bar code:CGTGCCGTAACTGGATTTTGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:4024
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:TACTCACCCTCCTTGGCGTA
Suggested primer 2:GCTCATTCATGCGTGCACAT