| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.043146 |
| Chromosome: | chromosome 16 |
| Location: | 6971429 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g672385 | HIS5 | (1 of 1) 2.6.1.9 - Histidinol-phosphate transaminase / Imidazolylacetolphosphate aminotransferase; Histidinol phosphate aminotransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCGCGCGGCCATAGCGCTGCTCGCCCGGGTTTCACCACGCCCTGTTG |
| Internal bar code: | GCCTGGAGTCTGGTCCCTTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3531 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTCCTAGCTCGACTCAGT |
| Suggested primer 2: | TGAACTTTCTGCATCGCCCT |