Insertion junction: CLIP2.043151_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.043151
Chromosome:chromosome 6
Location:8441827
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g308400 PTN1 (1 of 1) 3.1.3.16//3.1.3.48//3.1.3.67 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase // Protein-tyrosine-phosphatase / PTPase // Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase / Phosphatidylinositol-3,4,5-trisphosphate 3-phosphohydrolase; PTEN PI-3 phosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTCGGGTCGTCGCTTCCGCAGCGCCTTTGCCAGTTTGTCCAGCTGCGC
Internal bar code:GGCAAGCACTGAAGGTGATACT

Confirmation - LEAP-Seq

LEAP-Seq distance:3042
LEAP-Seq percent confirming:57.6923
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCCGGAACTCATCATAGA
Suggested primer 2:CTCTTCACATGCATGCGCTC