Insertion junction: CLIP2.043156_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.043156
Chromosome:chromosome 3
Location:511770
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145107 (1 of 3) K10406 - kinesin family member C2/C3 (KIFC2_3) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTATCAAAACTCGGACGCTGCAGTAGAGGCGCGGAAGCGGACGCTTCTGG
Internal bar code:GTACGCTTCTTGAATGTTGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:4816
LEAP-Seq percent confirming:98.4127
LEAP-Seq n confirming:62
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:63

Suggested primers for confirmation by PCR

Suggested primer 1:CCAATGGCGTGTGTGAAGTG
Suggested primer 2:TCTGACTTGCGAATGCCCAT