| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.043185 |
| Chromosome: | chromosome 6 |
| Location: | 8643986 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g309951 | (1 of 1) 3.2.1.18 - Exo-alpha-sialidase / Sialidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCCGGCTCAAAGATCTCAAACACCTCTGGCACCGTGTAGTTGACTTGC |
| Internal bar code: | AATGGTTCAGACGTGTGTAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2273 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCTCGCCCAACTAGCTCC |
| Suggested primer 2: | CATAGTCATCGCCCCAGGAC |