| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.043194 |
| Chromosome: | chromosome 3 |
| Location: | 5539557 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185100 | (1 of 1) K15698 - RING finger protein 121 (RNF121) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTTGGTACGGTGCACCTGCCCCGATCAGCCGCCAGAAGCCGGCGCAAA |
| Internal bar code: | TCAGTGGGGAACGAACTTTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3954 |
| LEAP-Seq percent confirming: | 96.9697 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAGCCGTGGTCGCCTATTG |
| Suggested primer 2: | TGACGCCCACAATTTTGCTG |