| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.043216 |
| Chromosome: | chromosome 16 |
| Location: | 2284000 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g801907 | CDS | ||
| lncRNA_TCONS_00176523 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATGGAGCAGGCGCAGCACCCAGGTCCAGGGTCTCCTGGTGGAGCTTCT |
| Internal bar code: | TATTGGGTCTATATACAGCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2358 |
| LEAP-Seq percent confirming: | 89.4737 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCACACGTACATCAATCC |
| Suggested primer 2: | GGGCAAACAGATAGCATGCG |