| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.043223 |
| Chromosome: | chromosome 10 |
| Location: | 1722260 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430100 | ELG22 | Exostosin-like glycosyltransferase 22; (1 of 6) IPR000742//IPR004263//IPR009030 - EGF-like domain // Exostosin-like // Insulin-like growth factor binding protein, N-terminal | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCACTGCCGCTCCAGTAAGTGCGGTGGGAGGGCCCCAACGCTGTGGCG |
| Internal bar code: | GCGGCGTGGTGCGAACATAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2194 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAATACGGCGTTTGATCCC |
| Suggested primer 2: | TGCAAGTGAAAATGCGGTGG |