| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.043238 |
| Chromosome: | chromosome 12 |
| Location: | 88585 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g483900 | (1 of 3) 3.6.3.2 - Magnesium-importing ATPase / Mg(2+)-importing ATPase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGATCGTGATGACCTCAGTCGTGCTGGGCACCGGCCTGCCCTTTGCCC |
| Internal bar code: | GCAAACCCTATGATTGTCCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2619 |
| LEAP-Seq percent confirming: | 84.4037 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 109 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCGAGGATGTCCATCAGC |
| Suggested primer 2: | CAAGGCCTCAGATGAGTGCA |