Insertion junction: CLIP2.043252_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.043252
Chromosome:chromosome 6
Location:6919419
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g296850 FAP81,DLEC1 (1 of 8) IPR008962 - PapD-like; Flagellar Associated Protein 81 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTCGCAACACAATACGATACGCTGTGATCCATCTTTCCACTTCTTGCC
Internal bar code:ATTTCCAAATTCATACCATGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:641
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AGCACAATCTCCCACCCATG
Suggested primer 2:GGAGCAGGTGACGTTCAAGA