Insertion junction: CLIP2.043256_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.043256
Chromosome:chromosome 16
Location:3719160
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g681657 (1 of 1) PF01344//PF13415//PF13418 - Kelch motif (Kelch_1) // Galactose oxidase, central domain (Kelch_3) // Galactose oxidase, central domain (Kelch_4) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGGGCCTGGGCCTGTGCTTGGGCCTGCTGGAGGGGCGGCTGCGGCGCT
Internal bar code:CATCAAACTCGCAGGCAGGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:2126
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGGCTACAGCTGTATCGG
Suggested primer 2:ATGCCCCAGAGGATGTAGGT