| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.043257 |
| Chromosome: | chromosome 17 |
| Location: | 4958768 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g735550 | (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGCTGAAGACGGGGGTGTGGAGCGGGGTCGGGGCTGCGACCGAAACGG |
| Internal bar code: | GATTAAAAGTTAGGAGCGATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6513 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACCTGGTATCGGCAGTCG |
| Suggested primer 2: | ATTCTGGCATGGAGGTGGTG |