| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.043258 |
| Chromosome: | chromosome 10 |
| Location: | 4535913 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451400 | NIT4,CNX1B,CNX1E | (1 of 1) 2.10.1.1//2.7.7.75 - Molybdopterin molybdotransferase // Molybdopterin adenylyltransferase; Molybdenum cofactor biosynthesis protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGCGAGGTCCCATAGGGTGCAGCTGACGCCCATACCCCAAGCAGACA |
| Internal bar code: | CGCGGATAGTACTACCGCGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4512 |
| LEAP-Seq percent confirming: | 94.5946 |
| LEAP-Seq n confirming: | 105 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 111 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTGGTGTGCTGGTGGTTT |
| Suggested primer 2: | CCTACATCACCACAGGCAGG |