| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.043259 |
| Chromosome: | chromosome 3 |
| Location: | 8463561 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g212529 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCATGGCAGCACAGTGGGCCGCGCGAAGGCCGCCTGCACCGCCGTCGT |
| Internal bar code: | GATCGTATACGGGCGGAAGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2319 |
| LEAP-Seq percent confirming: | 6.38298 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCCCATCACACATCCAGC |
| Suggested primer 2: | CGAGGGCTAATCGTGGACTC |