| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.043264 |
| Chromosome: | chromosome 12 |
| Location: | 1669628 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486450 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGTGCTTATGTCGGTCCGGTCCGACGATGTGCTTTGCTGCTACTGGG |
| Internal bar code: | TTTACACCGAAGGTCAGGGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1936 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCAAGCAACCAGGCAAC |
| Suggested primer 2: | CCCCCAATGCACATCCTTCT |