Insertion junction: CLIP2.043286_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.043286
Chromosome:chromosome 3
Location:8536868
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g211409 PPR15,mS106,MRPS106 Mitochondrial ribosomal protein S106, Chlamydomonas specific CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCTGCGGCTCCGTCGCCTCCTCCACCAGCTCTGTGCCCGGCTCAGCCG
Internal bar code:TCTTGACTGAAGGATACTCACT

Confirmation - LEAP-Seq

LEAP-Seq distance:2460
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTGCCACCATCTACGTGT
Suggested primer 2:CAACCTGGGTTCGCAACTTG