| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.043300 |
| Chromosome: | chromosome 17 |
| Location: | 1813114 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g709150 | HTB30,HTB3,HTB28 | (1 of 27) K11252 - histone H2B (H2B); Histone H2B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCACCTTCTCAAAGATGTCGTTGATGAAGCTGTTCATGATGGACATG |
| Internal bar code: | GCGGCTTTTTTGACAATCTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3173 |
| LEAP-Seq percent confirming: | 93.1034 |
| LEAP-Seq n confirming: | 81 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 87 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGGTCAAACAACCGCTC |
| Suggested primer 2: | ACACAGATGAATGCGGCAGA |