| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.043308 |
| Chromosome: | chromosome 16 |
| Location: | 552165 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692500 | (1 of 19) PTHR31251:SF1 - SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 10-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATGGAAAGCGCTCAACGCTAAGTAATCTATAGGAAGTAGGCTAAGGCC |
| Internal bar code: | CGTGCCCGGTTGTAACTTGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3015 |
| LEAP-Seq percent confirming: | 88.5246 |
| LEAP-Seq n confirming: | 216 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 244 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACGACAAGCTGCGGTCTT |
| Suggested primer 2: | TCTTGGCTCTACCTCCTCCC |