| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.043315 |
| Chromosome: | chromosome 2 |
| Location: | 1875686 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g087450 | (1 of 4) PTHR16255:SF4 - SPORULATION PROTEIN RMD8 | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTGCGTTCGAAGAGAGGTGCGGAGGTGATGGTGGTGGGCAACGGTGA |
| Internal bar code: | TACAAGTGCGCCGCAACTTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5120 |
| LEAP-Seq percent confirming: | 94.9495 |
| LEAP-Seq n confirming: | 94 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 99 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGTTCGTGCTTCGGTTG |
| Suggested primer 2: | GGTGAGTTTGAGCAGCTCCT |