| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.043317 |
| Chromosome: | chromosome 12 |
| Location: | 7664408 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555250 | NUOB14 | NADH:ubiquinone oxidoreductase B14 subunit; (1 of 1) K03950 - NADH dehydrogenase (ubiquinone) 1 alpha subcomplex subunit 6 (NDUFA6) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTATTAATCATACAAGTTATACAACACACCGCCGCTACACAACAGGTGA |
| Internal bar code: | AAACGAAATGAGTCTAATTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5594 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGACAGCTTAGCAACGCA |
| Suggested primer 2: | ACCTGCTCATCTTCAAGGGC |