Insertion junction: CLIP2.043319_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.043319
Chromosome:chromosome 3
Location:3326001
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g165950 (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGAAGCTCCTTCCCGAGGTAGGTGCGTGCGCGGGGCCTACGTACGGGTGG
Internal bar code:TTTACTGGCGAACTCAACTTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:4789
LEAP-Seq percent confirming:91.954
LEAP-Seq n confirming:80
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:87

Suggested primers for confirmation by PCR

Suggested primer 1:TACCCTCCTCCCCTTACTGC
Suggested primer 2:CACTCACGCACCCTTTTGTG