Insertion junction: CLIP2.043321_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.043321
Chromosome:chromosome 12
Location:8594348
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g548550 (1 of 1) PF05742 - Transport and Golgi organisation 2 (TANGO2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTGCGCAGCTCCGACCCTGTCGCCCCGCACACACACGCCAACCCCTAC
Internal bar code:GGAACGCGGTGGCGGTACACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1376
LEAP-Seq percent confirming:3.22581
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:60
LEAP-Seq n unique pos:62

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTCCAGAGACTGTGACAA
Suggested primer 2:CAACCTTGTCAAACTGGGCG