| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.043322 |
| Chromosome: | chromosome 2 |
| Location: | 3363031 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g098150 | XYK1 | (1 of 1) 2.7.1.17 - Xylulokinase / Xylulose kinase; Xylulose kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTCCGAGGCCTACCCCGACTGGCTGGCGCGGCAGGTGGGGATGAGGGC |
| Internal bar code: | ATAGTTATTGATTGGAGTAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1579 |
| LEAP-Seq percent confirming: | 72.0 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTAGGTCTCACCCAGTCGG |
| Suggested primer 2: | GGATGGTGGTACAAGTGGGG |