| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.043323 |
| Chromosome: | chromosome 12 |
| Location: | 5548877 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g530800 | (1 of 1) PTHR15887//PTHR15887:SF1 - FAMILY NOT NAMED // TRANSMEMBRANE PROTEIN 69 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGAAAGTGCCGATCCCAGTAACGTTGCCGTAACGCTACCATCCTAGT |
| Internal bar code: | GGTCAGAATATATAGAGGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3243 |
| LEAP-Seq percent confirming: | 87.9032 |
| LEAP-Seq n confirming: | 109 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 124 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGCCAGTGTACCCCAAG |
| Suggested primer 2: | TGTATACCGAACGAGCCGTG |