Insertion junction: CLIP2.043343_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.043343
Chromosome:chromosome 9
Location:51644
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTGGCCCTTTCGCCTCTTGCGCCACTGTGCGCCAGCTTCTCCTTGGCT
Internal bar code:AAAGTGGCGGCTACATGGCTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1246
LEAP-Seq percent confirming:31.25
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TCTCGTTCTGTGTTGGCTCC
Suggested primer 2:CGTGAGATGGAGTGCTTGGT