| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.043343 |
| Chromosome: | chromosome 15 |
| Location: | 6597 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g801670 | (1 of 1) IPR010254 - B12-dependent dehydratases, beta subunit | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGCTGTCTGTCGCAGAGGGCAAGGGAGAGAGGGACAGGAGGCCGCCCC |
| Internal bar code: | AGAAGTAAGGGTGTGGTTAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 266 |
| LEAP-Seq percent confirming: | 3.33333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTCTGGAATGCAGCGAAT |
| Suggested primer 2: | ATGTGACTGCCCAGTTCCTG |