| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.043354 |
| Chromosome: | chromosome 12 |
| Location: | 5719376 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532300 | APG10,ATG10 | Autophagy-related protein; (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTACGTAGTAGAAATGAACCTCAAACTCGCGAGTTCCTTCACCTATTC |
| Internal bar code: | TTAGCGATTTGCGGGCCTCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 360 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCCCGTAACGTTTTGTC |
| Suggested primer 2: | GTTCTGCTGCTGCTAGGTGA |