Insertion junction: CLIP2.043356_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.043356
Chromosome:chromosome 13
Location:1384566
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g571850 APC10 Anaphase promoting complex subunit 10; (1 of 1) K03357 - anaphase-promoting complex subunit 10 (APC10, DOC1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGCATGCTGGATCTTCACGTCGCCACGCACACACCACACACACACACAC
Internal bar code:TAGGTTTTAATTGTTGTGTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:3065
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GATTACTCCACACGGGCACA
Suggested primer 2:AACACGCACCTGTCGTATGT