Insertion junction: CLIP2.043356_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.043356
Chromosome:chromosome 13
Location:1384568
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g571850 APC10 Anaphase promoting complex subunit 10; (1 of 1) K03357 - anaphase-promoting complex subunit 10 (APC10, DOC1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAGCGCGGCCATCATGCGCAGTAAATCCGACAACCACACCTCAGGACC
Internal bar code:CTAACCTGGTGGGGGGAAGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1217
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AACACGCACCTGTCGTATGT
Suggested primer 2:GATTACTCCACACGGGCACA