Insertion junction: CLIP2.043394_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.043394
Chromosome:chromosome 3
Location:6664011
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g195300 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCCAGCATCGAGGCCTCGACGCCATCGAGCTCGCCACCGAACTTGTCA
Internal bar code:GCTTCAGGAACAAATACGGAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:3887
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTGTTCAAGAAGCTGCAG
Suggested primer 2:GAAACAGCAGCAACCCAAGG