Insertion junction: CLIP2.043401_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.043401
Chromosome:chromosome 16
Location:6127970
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g677150 (1 of 2) 2.4.1.122 - Glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTAGGATCGAGCGCTTGGGCGCGCGCGCGCTTGTCATTACCCTACAAG
Internal bar code:TCCGAATTAGATGCGTGCACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1847
LEAP-Seq percent confirming:96.1538
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:CAATAAACCGGATGCGGCTG
Suggested primer 2:TGTGTCACCGTAGTGTCTGC