Insertion junction: CLIP2.043408_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.043408
Chromosome:chromosome 1
Location:3972649
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g025983 protein of unknown function with DUF1399 domain; (1 of 11) PF07173 - Protein of unknown function (DUF1399) (DUF1399) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCGCTCATTTGGACACCGCTTTCCACTTTAGTCGCTCATACTAATATA
Internal bar code:AGTATTTTCTTTGATTAGGTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1116
LEAP-Seq percent confirming:11.3208
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:47
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:TACCGCTGCGAGAAGTTTGT
Suggested primer 2:GATGTCCAGAGCGCGATGTA