| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.043411 |
| Chromosome: | chromosome 8 |
| Location: | 52131 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358200 | ACV4 | protein kinase; (1 of 6) PTHR23257//PTHR23257:SF529 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGACAATGGAGCCGTACGTGTCGTAGTTGAGGCCCATCTCCGGGAAGTC |
| Internal bar code: | CAGAATGATTGAGGATCAAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3570 |
| LEAP-Seq percent confirming: | 95.6522 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGACTATTTGTGCGCGAAT |
| Suggested primer 2: | CGTGTCATACCTCACCCCAC |