Insertion junction: CLIP2.043420_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.043420
Chromosome:chromosome 12
Location:3441132
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g497500 SNE7 (1 of 8) PTHR10366:SF411 - HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 173 PROTEIN; Cinnamoyl-CoA reductase/flavanone 4-reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGTGCCGACCCATGGCTGGACAGAAGCAAGCCGGGGCACCCGTATTGG
Internal bar code:ATAAGTGGTTTAGGCGGTCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:3977
LEAP-Seq percent confirming:7.52688
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:86
LEAP-Seq n unique pos:93

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAAAAGGGTTTGGTGGCA
Suggested primer 2:AAAGGTCTGCGTCACTGGAG