| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.043460 |
| Chromosome: | chromosome 2 |
| Location: | 8281444 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g143350 | PDE6 | (1 of 20) 3.1.4.17 - 3',5'-cyclic-nucleotide phosphodiesterase / Cyclic AMP phosphodiesterase; 3'%252C5'-cyclic-nucleotide phosphodiesterase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGCCGCCACGTGTCAAACTGCCAGCCGTCCGCGCCCGCCAGCACCTTC |
| Internal bar code: | TTGTCACAATCTTCCGAGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2155 |
| LEAP-Seq percent confirming: | 93.8775 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCAGCTTCGCATTCACG |
| Suggested primer 2: | AGTGCTGAGACACAGGCATC |