| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.043471 |
| Chromosome: | chromosome 1 |
| Location: | 5302860 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037300 | (1 of 140) IPR002048 - EF-hand domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTCACCACCGGCACCGGCGCCAGCCCGACCTCCGCCTCCTGCCAGCG |
| Internal bar code: | CTACATTGGTTTATTTCCAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4748 |
| LEAP-Seq percent confirming: | 86.9565 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATACAACACGCGAGCATGC |
| Suggested primer 2: | CCCAGCTCCAGGCATATCTG |