Insertion junction: CLIP2.043474_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.043474
Chromosome:chromosome 17
Location:5270749
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACAGGCTGCTTGGGGAGCTGTATTGCCCAGCAACTCGGGCCAGACGGAG
Internal bar code:TTATACATGCCGGACCTAATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2791
LEAP-Seq percent confirming:82.2222
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:TCCCTCTCTCTTCAGTCCCG
Suggested primer 2:GTGCTGTGATGCCCTACCTT