| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.043482 |
| Chromosome: | chromosome 1 |
| Location: | 3998738 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g026250 | AGA1 | Alpha-galactosidase; (1 of 1) 3.2.1.22 - Alpha-galactosidase / Melibiase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTGCGTCTGCGGTATAAGGGTTGGCCAGGCGGCGAGGAGAACCGCGGA |
| Internal bar code: | TGGCATATAGGAAGTTTAAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2261 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCGTTGTGTTTGACAGAC |
| Suggested primer 2: | TGAGGTTCATGTCGCTGCAT |