| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.043487 |
| Chromosome: | chromosome 14 |
| Location: | 2636984 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g625350 | MAE17 | MATE efflux family protein; (1 of 3) PTHR11206:SF95 - MULTI ANTIMICROBIAL EXTRUSION FAMILY PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCGAGCCCAAAGATATAGTCCCAGTAGCCAAGGAATCGCCGCCCAACC |
| Internal bar code: | ACCCGAGATTGATTAAGTGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 660 |
| LEAP-Seq percent confirming: | 96.1538 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATTCCGCTTCACAGCTA |
| Suggested primer 2: | TACTGGTTTTGCGATCGGCT |